The present study is concerned with both in-vitro assessment of anti-hemolytic and protein denaturation activity of hydroethanolic extract of Calendula officinalis, Ficus bengalensis and Juglans regia. Total flavonoids and phenolics also were determined by using aluminum nitrate and Folin–Ciocalteu colorimetric methods using quercetin and tannic acid as reference standard respectively. The antioxidant capacity of sample was assessed through reducing power assay, DPPH-scavenging effect IC50 values of extracts being 29.012±1.3429 (µg/ml), 33.739±0.923 (µg/ml) and 40.231±1.323 (µg/ml) respectively. The maximum anti-hemolytic activity 120.542±0.438 (µg/ml), 148.839±1.482 (µg/ml) and 214.190±1.584 (µg/ml). The findings infer that Calendula officinalis substantiates the promising in-vitro antioxidant, antihemolytic and protein denaturation activity in the tested models.
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